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La Sistina is located in Laguna del Monte, Guaminí, a town on the southwest of Buenos Aires Province and approximately 480 km away from the Autonomous City of Buenos Aires, 190 km from Bahía Blanca and 70 km from Coronel Suárez. Access to the island can be made by speedboat on a beautiful ten minutes trip, or by plane, since the island has a 1,200 m airstrip.